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La conférence Olszewski du Neuro, créée en 1986, rend hommage au Dr Jerzy Olszewski (1913–1964), un neuroanatomiste et neuropathologiste pionnier. Invité au Neuro par le Dr Wilder Penfield en 1948, Olszewski a collaboré à l’ouvrage fondamental Cytoarchitecture of the Human Brain Stem, contribuant à l’avancement de la compréhension des structures du tronc cérébral et établissant son héritage dans le domaine des neurosciences.

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Xiaowei Zhuang 

Howard Hughes Medical Institute, Harvard University

Abstract: Cell and tissue functions arise from the coordinated activities of thousands of different genes. Understanding these functions requires imaging at the genome scale, which advances knowledge in many areas of biology, from the regulation of gene expression in cells to the development of cell fate and the organization of cell types in complex tissues. A single-cell genome-scale imaging method, multiplexed error-robust fluorescence in situ hybridization (MERFISH), was developed to enable spatially resolved single-cell transcriptomics, epigenomics, 3D-genomics, and functional genomics. This method allows for single-cell gene-expression profiling in intact tissues, facilitating the identification, spatial mapping, and functional investigation of distinct cell types within those tissues. Using this approach, molecularly defined, spatially resolved, and functionally annotated cell atlases of the brain have been created. This lecture will describe the MERFISH technology and its applications, with a particular focus on mapping the molecular, spatial, and functional organizations of distinct cell types in the brain.