Dr. Mari Kaartinen
Associate Professor - Faculty of Dentistry, Division of Biomedical Sciences
Associate Professor - Faculty of Medicine, Department of Anatomy and Cell Biology
Associate Member聽- Department of Medicine
Dr. Kaartinen鈥檚 research is focused on understanding how extracellular environment regulates cell behavior in bone and fat tissues. We are specifically focused on investigating how extracellular matrix proteins and their posttranslational processing by transglutaminase enzymes, modulates mesenchymal stem cell survival, function and differentiation to adipocytes and osteoblasts and how this links to pathophysiology of osteoporosis, defects in energy metabolism and adipogenesis. We also investigate monocyte and macrophage differentiation and their interaction with mesenchymal stem cell lineage cells in the bone marrow. Our expertise involves transgenic animal models and animal models of disease, cell and matrix biology, biochemistry and chemical biology.
Dr. Kaartinen received her MSc in Organic Chemistry from University of Jyvaskyla (Finland) and PhD in Biochemistry from the University of Kuopio (currently University of Eastern Finland) in 1999. She completed her postdoctoral training at 成人VR视频 and joined the Faculty of Dentistry and Faculty of Medicine at 成人VR视频 in 2002.
Key words:聽extracellular matrix, bone marrow, mesenchymal stem cells
- Role of transglutaminases in bone remodeling
- Role of FXIII-A/F13A1 in energy metabolism in mice and humans
- Plasma fibronectin assembly in bone and adipose tissue
- Exercise, bone and energy metabolism
- Transglutaminase-mediated assembly of plasma fibronectin
- Plasma fibronectin, cell adhesion and differentiation
Active Research Grants
- CIHR (2019-2024) Regulation of bone remodeling by transglutaminases
- CIHR (2017-2022) Role of FXIII-A and plasma fibronectin in etiology of obesity and type 2 diabetes
Peer-reviewed Articles
Mousa A, Cui C, Song A, Myneni VD, J. Li, Murshed M, Melino G,聽Kaartinen MT聽(2017). Transglutaminases Factor XIII-A and TG2 regulate resorption, adipogenesis and plasma fibronectin homeostasis in bone and bone marrow. Cell Death Diff.聽 24(5):844-854
Hoac B, Nelea V, Jiang W,聽Kaartinen MT, McKee MD (2017) Mineralization-inhibiting effects of transglutaminase-crosslinked polymeric osteopontin. Bone. 101:37-48
Myneni VD,聽Kaartinen MT聽(2016). Factor XIII-A deficient mice show signs of metabolically healthy obesity on high fat diet. Sci Rep.聽6:35574.
Myneni VD,聽Kaartinen MT聽(2015). Transglutaminase 2 - A novel inhibitor of adipogenesis.聽Cell Death & Dis.聽6:e1868.
Wang S,聽Kaartinen MT聽(2015). Cellular Factor XIIIA transglutaminase localizes in caveolae and regulates caveolin-1 phosphorylation, homo-oligomerization and c-Src signaling in osteoblasts.聽J. Histochem Cytochem.聽63(11):829-41.
Cui C,聽Kaartinen MT聽(2015). Monoamines serotonin, inhibit plasma fibronectin assembly into extracellular matrix by acting as a competitive inhibitor to its transglutaminase mediated crosslinking.聽Bone.聽72:43-52.
Addison WN, Nelea V, Chicatun F, Chien Y-C, Vali H, Tran-Khanh N, Buschmann MD, Nazhat SN,聽Kaartinen MT, Vali H, Tecklenburg M, Franceschi RT and McKee MD (2015). Extracellular matrix mineralization in murine MC3T3-E1 osteoblast cultures: An ultrastructural, compositional and comparative analysis with mouse bone.聽Bone.聽71C:244-256. 聽
Dargahi M, Nelea V, Mousa A, Omanovic S, Kaartinen MT聽(2014) Electrochemical modulation of plasma fibronectin surface conformation enables filament formation and control of endothelial cell-surface interactions.聽RSC Adv.聽4 (88): 47769 - 47780.
Myneni VD, Hitomi K,聽Kaartinen MT聽(2014). Factor XIII-A transglutaminase acts as a switch between preadipocyte proliferation and differentiation.聽Blood.聽124(8):1344-53.
Wang S, Cui C, Hitomi K,聽Kaartinen MT聽(2014). Detyrosinated Glu-tubulin is a substrate for cellular Factor XIIIA transglutaminase in differentiating osteoblasts.聽Amino Acids.聽46(6):1513-26.
Ka K, Nicolau B, Henderson M, Tran S,聽Kaartinen MT, Myneni VD, Rousseau M-C (2014). Circulating uncarboxylated osteocalcin and gingival crevicular fluid tumour necrosis factor-伪 in children.聽J. Clin. Periodont.聽41(5):467-72.
Eckert R,聽Kaartinen聽 MT,聽Nurminskaya M, Belkin A, Colak G, Johnson GCW, Mehta K (2014). Transglutaminase regulation of cell function.聽Physiol. Reviews.聽94, 383-417.
Cui C, Wang S, Myneni VD, Hitomi K,聽Kaartinen MT聽(2014). Transglutaminase activity is required for stabilization and conversion of plasma FN into permanent osteoblast matrix.聽Bone聽59, 127-138.
Javaid M,聽Kaartinen MT聽(2013) Mesenchymal stem cell-based bone tissue engineering. Review.聽Int.聽Dental Journal of Students鈥� Research.1 (3), 24-35.
Dastjerdi AK, Pagano M, McKee MD,聽Kaartinen MT聽and Barthelat F (2012). The cohesive behavior of soft biological "glues": Experiments and modeling.聽Acta Biomater.聽8: 3349鈥�3359.
Piercy-Kotb SA, Mousa A, Al-Jallad HF, Myneni VD, Chicatun F,聽Nazhat SN,聽Kaartinen MT聽(2012). Factor XIIIA expression and secretion in osteoblast cultures is regulated by extracellular collagen network and MAP kinase pathway.聽J Cell Physiol.聽227(7):2936-46.
Chicatun F, Pedraza CE, Ghezzi CE, Marelli B,聽Kaartinen MT, McKee MD, Nazhat SN (2011).Bone extracellular matrix mimicking dense collagen/chitosan gel scaffolds.聽Biomacromolecules.聽12(8):2946-56.
McKee MD, Pedraza CE,聽Kaartinen MT聽(2011).聽Osteopontin and wound healing in mineralized tissues.聽Cells, Tissues, Organs.聽194:313-319.
Al-Jallad HF, Myneni VD, Piercy-Kotb SA, Chabot N, Mulani A, Keillor JW,聽Kaartinen MT聽(2011). Plasma membrane Factor XIIIA transglutaminase activity regulates osteoblast matrix secretion and deposition via affecting microtubule dynamics.聽PLoS ONE.聽6 (1):e15893.
Forsprecher J, Wang Z, Goldberg HA,聽Kaartinen MT聽(2011). Transglutaminase-mediated oligomerization promotes osteoblast adhesive properties of osteopontin and bone sialoprotein.聽Cell Adh. Migr.聽5 (1) 1-8.
Nelea V,聽Kaartinen MT聽(2010). Periodic beaded-filament assembly of fibronectin on negatively charged surfaces.聽聽J. Struct. Biol.聽170, 50鈥�59.
Nakano Y, Forsprecher J and聽Kaartinen MT聽(2010). Regulation of ATPase activity of transglutaminase 2 by MT1-MMP - implications to mineralization of osteoblast cultures聽J. Cell Physiol.聽2010 223(1):260-269
Forsprecher J, Wang Z, Nelea V,聽Kaartinen MT聽(2009). Enhanced osteoblast adhesion on transglutaminase 2 - polymerized fibronectin.聽Amino Acids.聽36(4): 747-753.
Pedraza CE, Nicholcheva LG,聽Kaartinen MT, Barralet JE, and McKee MD (2008). Osteopontin functions as an opsonin and facilitates phagocytosis of hydroxyapatite-coated microspheres by macrophages:聽 Implications for bone wound healing.聽 Bone.聽43(4):708-16.
Hubmacher D, El-Hallous EI, Nelea V,聽Kaartinen MT, Lee ER, and Reinhardt DP (2008). Oligomerization of the C-terminus of fibrillin-1 provides the basis for microfibril assembly.聽聽PNAS.聽105聽(18): 6548-6553.
Keillor JW, Chica RA, Chabot N, Vinci V, Pardin C, Fortin E, Gillet SMFG, Nakano Y,聽Kaartinen MT, Pelletier JM and Lubell WD (2008). The bioorganic chemistry of transglutaminase: From mechanism聽to inhibition and聽engineering.聽Can. J. Chem.聽86聽(4): 271-276.
Nelea V, Nakano Y,聽Kaartinen MT聽(2008).聽 Size distribution and molecular association of plasma fibronectin and fibronectin multimers crosslinked with transglutaminase 2. Protein J.聽27(4): 223-233.
Nakano Y, Addison WN,聽Kaartinen MT聽(2007).聽 ATP mediated mineralization of MC3T3-E1 osteoblast cell cultures.聽聽Bone.聽聽41(4): 549-561.
Kaartinen MT, Schinke T, Murshed M, Karsenty G, McKee MD. Osteopontin upregulation and polymerization by transglutaminase in the calcified arteries of聽 matrix Gla protein-null mice.聽聽Submitted to J.聽 Histochem.聽 Cytochem.
Addison WN, Azari F, Sorensen ES,聽Kaartinen MT, McKee MD. Pyrophosphate inhibits mineralization of osteoblast cultures by binding to mineral, upregulating osteopontin and by inhibiting alkaline phosphatase activity.聽 Submitted to J. Biol. Chem.
McKee MD, Addison W,聽Kaartinen MT聽(2006) Hierarchies of extracellular matrix and mineral organization in bone of the craniofacial complex and skeleton.聽聽Cells, Tissues, Organs.聽181聽(3-4), 176-88.
Al-Jallad HF, Nakano Y, Chen JLY, McMillan E, Lefebvre C,聽Kaartinen MT聽(2006). Transglutaminase activity regulates osteoblast differentiation and matrix mineralization in聽 MC3T3-E1 osteoblast cells.聽Matrix Biology.聽25,聽135-148.
Nourminskaya M,聽Kaartinen MT聽(2005) Transglutaminases in mineralized tissues. In press (Frontiers in Biosciences)聽
Lefebvre C, Al-Jallad HF and聽Kaartinen MT聽(2005) Transglutaminase 2 and Factor XIIIa expression, activity and localization in mineralizing osteoblast cultures聽Proc. of VIIIth Int鈥檒. Conf. Chem. Biol. Mineralized Tissues, Banff, AL, October.聽In Press聽
Kaartinen MT, Wei Sun, S, Kaipatur N, McKee MD (2005) Transglutaminase crosslinking of SIBLING proteins in teeth. J Dent. Res. 84(7):607-612
El-Maadawy S,聽Kaartinen MT, Schinke T, Murshed M, Karsenty G, McKee MD (2003) Cartilage formation and calcification in arteries of mice lacking matrix Gla protein. Connect. Tissue Res. 44 (Suppl. 1), 1-7
Kaartinen MT, El-Maadawy S, R盲s盲nen NH, McKee MD (2002) Transglutaminase and its substrates in bone. J. Bone Miner. Res. 12, 2161-2173
Goldsmith HL, McIntosh FA, Labrosse JM,聽Kaartinen MT, M盲enp盲盲 PH, McKee MD (2002) Homotypic interactions of soluble and immobilized osteopontin. Ann. Biomed. Eng. 30, 840-850
Luedtke CC, McKee MD, Cyr D, Gregory M,聽Kaartinen MT, Mui J, Hermo L (2002) Osteopontin expression and regulation in the testis, efferent ducts and epididymis of rats during postnatal development through to adulthood. Biol. Reprod. 66, 1437-1448
Nousiainen M, Derrick PJ,聽Kaartinen MT, M盲enp盲盲 PH, Rouvinen J, Vainiotalo P (2002) A mass spectrometric study of metal-binding to osteocalcin. Chem. Biol. 9, 195-202
Kaartinen MT, Pirhonen A, Linnala-Kankkunen A, M盲enp盲盲 PH (1999) Transglutaminase treated osteopontin exhibits increased collagen binding properties. J. Biol. Chem. 274, 1729-1735
Kaartinen MT, Pirhonen A, Linnala-Kankkunen A, M盲enp盲盲 PH (1999) Transglutaminase catalyzed cross-linking of osteopontin is inhibited by osteocalcin. J. Biol. Chem. 272, 22736-22741
Lappalainen K, Kolehmainen E,聽Kaartinen MT, Kauppinen R, Seppal盲 R, Vatanen V (1994) Substituted methyl-5-cholan-24-oates, II-13C-spectral assignment. Magn. Reson.Chem. 32, 786-788
Kolehmainen E,聽Kaartinen MT, Kauppinen R, Kotoneva J, Lappalainen K (1994) Substituted methyl 5b-cholan-24-oates, I-17O NMR spectral characterization. Magn. Reson.Chem. 32, 441-445
Book Chapters
Khayer Dastjerdi AK, Pagano M聽Kaartinen MT, McKee MD and Barthelat F (2013) Direct Measurements of the Cohesive Behavior of Soft Biological Interfaces.聽Book Chapter/Conference Proceedings of the Society for Experimental Mechanics Series,聽Mechanics of Biological Systems and Materials, Volume 5: 207-215
McKee MD, Murshed M,聽Kaartinen MT聽(2011) Extracellular matrix of craniofacial bone. In:聽Mineralized Tissues in Oral and Craniofacial Science: Biological Principles and Clinical Correlates.聽 Laurie McCauley and Martha Somerman, eds.聽Wiley-Blackwell.
McKee MD,聽Kaartinen MT聽(2002) Regulation of biomineralization by proteins and their assembly into extracellular matrices: Bone and the osseointegrated implant interface. In: Aging, Osteoporosis and Dental Implants. Zarb G, Lekholm U, Albrektsson T and Tenenbaum H, eds. Quintessence Pub. Co., Inc., Carol Stream, IL. p. 191-205
Kaartinen MT, Pirhonen A, Linnala-Kankkunen A, M盲enp盲盲 PH (2000) Tissue transglutaminase treatment increases osteopontin's collagen binding properties and results in affinity for tropoelastin. In: Chemistry and Biology of Mineralized Tissues, eds. Robinson C, Boskey A, and Goldberg M, American Academy of Orthopaedic Surgeons, New York, Chapter 37, pp. 229-232 (peer reviewed article)