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Systems Approaches to Decipher the Molecular Mechanisms of Evolutionary Innovation

Anne-Ruxandra Carvunis, University of Pittsburgh
Tuesday October 17, 12-1pm
Zoom Link:听
In Person: 550 Sherbrooke, Room 189

Abstract:听The yeast genome contains more than 200,000 open reading frames (ORFs), of which ~5,000 are annotated with confidence as protein-coding genes. Most yeast ORFs are short and lack evidence of conservation between closely related species or between isolates. These sequence properties are consistent with the notion that these ORFs transiently emerge and then disappear with random mutations in the absence of selection. As a result, these ORFs remain unannotated. However, we show by integrative ribosome profiling analyses that up to 20,000 unannotated yeast ORFs are translated. Through systematic analyses of gain- and loss-of-function mutants, high-content microscopy and integrative transcriptomics, we are uncovering evidence that transiently emerging ORFs carry a surprisingly profound impact on cell biology and fitness despite their seemingly random evolutionary patterns. We discuss the implications of our findings for the systems biology of evolutionary innovation.