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Call to all ³ÉÈËVRÊÓƵ Labs: Urgent need for reagents for use in COVID-19 testing

Published: 20 March 2020

The Public Health AgencyÌýofÌýCanada has an urgent need for reagents forÌýuse inÌýCOVID-19 testing.ÌýÌýThe National Microbiology Lab (NML) is specifically looking for items on the list in the table below, as well as any other RNA extraction reagents.

Please connect directly with Mette Cornelisse, Office of Chief Science Officer (mette.cornelisse [at] canada.ca) and Rita Finley, Office of Chief Science Officer (rita.finley [at] canada.ca).

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PCR

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1 ABI TaqMan Fast Virus 1-step Master Mix 1 x 10mL
2 TaqPath 1-Step Multiplex MM Thermofisher scientific (contains Mustang Purple reference dye)
3 TAqPath 1-step multi NO ROX Thermofisher scientific (contains no reference dye)
4 LC Multiplex RNA virus Master Roche
5 LightMix® Modular Wuhan CoV E-gene assay Roche
6 LightMix® Modular Wuhan CoV RdRp-gene as Roche
7 Luna® Universal Probe One-Step RT-qPCR Kit (New England Biolabs (NEB)) New England Biolabs
8 geneXpert COVID-19 RUO Ìý
Ìý Ìý Ìý
Ìý Extractions Ìý
9 ABI 5x MagMAX-96 viral isolation kit Ìý
10 Ìý Carrier RNA /
11 Ìý MagMax Lysis/Binding Solution concentrate
12 Ìý Wash solution #1 concentrate
13 Ìý 0.1nM EDTA in DEP-C Treated water
14 Ìý Wash solution #2 ConcentrateÌý
15 Ìý 7.Ìý Tip Comb KF96 F/DWMAGNET CS100 / CA83007-594 - SKU for KingFisher Flex 96 tip comb for DW magnets
16 Ìý Plate PP 96 Deep V-BTM 2mL (Deep well) / CA11388-566 - sku forÌý Microtiter deep well 96 plate, V-bottom
17 Ìý 96 well KF 200uL PK48 / CA83007-596- sku for KingFisher 96 KF plate (200 µl)
18 MagMAXâ„¢-96 Viral RNA Isolation Kit from Thermo fisher Ìý
19 Biosprint 96 One-For-All Vet Kit (384) from Biovet INDICAL Ìý
20 Biomerieux Easymag and emag NUCLISENS easyMAG Lysis Buffer (pre dispensed in 2 ml tubes)
21 Ìý NUCLISENS easyMAG Wash Buffer 1
22 Ìý NUCLISENS easyMAG Wash Buffer 2
23 Ìý NUCLISENS easyMAG Wash Buffer 3
24 Ìý NUCLISENS easyMAG Silica
25 Ìý NUCLISENS easyMAG Lysis Buffer (1 liter, self dispense)
26 Ìý NUCLISENS easyMAG disposables
27 MagNA Pure Compact Nucleic Acid Isolation Kit IÌýÌýÌýÌý ÌýÌý Ìý
28 LightCycler® Multiplex RNA Virus Master: Ìý
29 Magna Pure consumables REAGENT MAG NA PURE LC TOTAL ISOLATION KIT
30 Ìý REAGENT CAPILLARIES LC
31 Ìý REAGENT MAG NA PURE LC TUB LID SEAL
32 Ìý REAGENT MAG MA PURE LC TUB MEDIUM M20
33 Ìý REAGENT MAG NA PURE LC TUB LARGE
34 Ìý REAGENT MA NA PURE LC TUB LID SMALL MED
35 Ìý REAGENT MAG NA PURE LC TUB LID LARGE
36 Ìý REAGENT MAG NA PURE LC SAMPLE CART.
37 Ìý REAGENT MAG NA PURE LC PROCESS CART.
38 Ìý REAGENT MAG NA PURE LC TIP STAND
39 Ìý REAGENT MAG NA PURE LC CART. SEAL
40 Ìý REAGENT MAG NA PURE LC REACTION TIPS
41 Qiagen QiaSymphony QIAsymphony DSP Virus Pathogen mini kit (Buffer AVE, Carrier RNA),
42 Ìý Buffer ATL,
43 Ìý 8-well Sample Cartridge
44 Ìý 8-Rod Cover
45 Ìý Filter tip 200 ul
46 Ìý Filter tip 1500 ul
47 Invitrogen SuperScript III Platinum 500 rxn One Step Real time kitÌýÌýÌýÌýÌýÌýÌýÌý Ìý

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