The rates listed below are for instrument time for independent users after staff training. Add the technical time hourly rate for assistance from FEMR staff, including training.
| Electron Microscope | Regular Hours (8:00聽 鈥� 18:00) | Afterhours and Weekends |
|---|---|---|
| FEI Titan Krios 300 kV Cryo-S/TEM | $1,000 day-1; SPA applications | |
| $750 day-1; Cryo-ET applications | ||
| $500 day-1; Screening(*) | ||
| FEI Tecnai G2 F20 200 kV Cryo-S/TEM | $70.00 hr-1 | $40.00 hr-1 |
|
Thermo Scientific Talos F200X for Materials Science |
$70.00 hr-1 | $40.00 hr-1 |
| FEI Tecnai G2 Spirit 120 kV Cryo-TEM | $60.00 hr-1 | $40.00 hr-1 |
| FEI Tecnai 12 120 kV TEM | $60.00 hr-1 | $30.00 hr-1 |
| FEI Helios Nanolab 660 DualBeam |
$100.00 hr-1 |
$100.00 hr-1 |
| FEI Quanta 450 FE-ESEM | $60.00 hr-1 | $30.00 hr-1 |
| Hitachi Ethos NX5000 FIB-SEM | $100.00 hr-1 | $100.00 hr-1 |
| FEI CorrSight Digital Fluorescent Light Microscope | Live Cell Module $40.00 hr -1 | |
| Cryo Module $60.00 hr -1 | ||
(*)If the screening day evolves into a data collection day, the cost is $1000 day-1.聽 Data collection projects approved by the Krios Committee have priority over screening projects.
| Sample Preparation Equipment | Fees | |
|---|---|---|
| Bal-Tec BAF060 Freeze Fracture System | $40.00 hr-1, min. 3 hrs | |
| Bal-Tec BAF030 Jet Freezer | $35.00 hr-1 | |
| Cressington 208A Carbon Coater | $20.00 hr-1 | |
| Edwards Carbon Coater Model 306A | $25.00 hr-1 | |
| FEI Vitrobot Mark IV | $35.00 hr-1 | |
| Gatan Precision Ion Polisher System (PIPS) | $35.00 hr-1 | |
| Leica Microsystems Ultracut UCT Ultramicrotome | $30.00 hr-1 | |
| Leica Microsystems EM PACT2 High-Pressure Freezer | $35.00 hr-1 + HPF consumables at cost | |
| Leica Microsystems EM AFS2 | $35 day-1 | |
| Leica Microsystems Critical Point Dryer Model CPD030 | $30.00 hr-1 min. 2 hrs | |
| Leica Microsystems EM ACE600 Sputter Coater | $25.00 run-1 | |
| Technical assistance | $70.00 hr-1 | |
Typical costs for electron microscopy laboratory services. Actual costs may depend upon the project and the type of sample(s).
| Services | Fees |
|---|---|
| Technical time | $70.00 hr-1 |
| Consultation on experimental design. | No charge first hour hr-1; $70 for each additional hr. |
| Training on TEM | $140.00 hr-1; min. 3 hrs |
| Training on SEM | $130.00 hr-1; min. 2 hrs |
| Conventional sample preparation for TEM (fixation and embedding/) | $450.00 (up to ~12 samples + $50.00 for each additional sample) + ~10% material fee (fixatives, buffers, resins, etc.) |
| Post-embedding sample preparation for TEM (LR White) | $300.00 (up to ~10 samples) + ~10% material fee (fixatives, buffers, resins, etc.) |
| Ultramicrotomy | $75.00听丑谤-1 + cost of consumables |
| Cryo-ultramicrotomy | $120 hr-1 + cost of consumables |
| Post-embedding immunogold labeling | $350.00(#) |
| Cryo-immunogold labeling | $450.00(#) |
| Negative staining | $75.00听丑谤-1 + cost of consumables |
| Cryo-sample preparation (grid plunge freezing or Vitrobot) | $105.00 hr-1 + cost of consumables |
| Freeze-fracture preparation + TEM imaging | $500.00 |
| Fixation and CPD for imaging in SEM | $275 + cost of consumables (up to 6 samples) |
| Specimen mounting and sputter coating for SEM | $50 run-1 + cost of consumables |
| Image Processing | $75 hr-1 |
(#) Consult with staff for聽details
For more information about services or the availability of equipment E-mail kelly.sears [at] mcgill.ca.
Publication/Authorship Policy
The FEMR provides services for electron microscopy on a fee-for-service basis. Basic processing and microscopy are the responsibility of the staff of the FEMR. Interpretations of resulting data are the responsibility of the submitting investigator. The FEMR should be acknowledged in publications in which microscopy results are utilized. Co-authorship on resulting publications should be considered when projects brought to the FEMR require significant additional scientific or intellectual input from FEMR staff.