Stefano Stifani, PhD
Research in the Stifani laboratory focuses on understanding the biology of neural stem/progenitor cells in the healthy and diseased nervous system, as well as harnessing the potential of human induced pluripotent stem cells to establish informative experimental model systems to study diseases and disorders of the nervous system.
Thiry L, Pulimood NS, Tang Y, Stifani S. Dysregulated expression of inflammasome and extracellular matrix genes in C9orf72-ALS/FTD microglia. ASN Neuro. 2025;17(1):2542998. doi: 10.1080/17590914.2025.2542998.
Carrier I, Diez E, Piscopo VEC, Bechstedt, S. van Bokhoven H, Srour M. Berghuis A, Stifani S, Yamanaka Y, McInnes RR. The microtubule-binding protein EML3 is required for mammalian embryonic growth and cerebral cortical development; Eml3 null mice are a model of cobblestone brain malformation. 2025. eLife14:RP107102.
Thiry, L., Sirois, J., Durcan, T.M. Stifani, S. Derivation and Culture of Enriched Phrenic-Like Motor Neurons From Human iPSCs. Bio Protoc. 2025 Jul 5;15(13):e5363. doi: 10.21769/BioProtoc.5363. eCollection 2025 Jul 5.
Deluc T, Ase A, Dorion MF, Maussion, G, Tang Y, Lo RTM, Shlaifer I, Piscopo VE, Durcan TM, Stifani S, Seguela P. Adrenergic control of P2Y6 receptor-dependent phagocytosis in rodent and human microglia. Glia. 2025 Jul 15. doi: 10.1002/glia.70054.
Megagiannis P, Mei Y, Yan RE, Yuan L, Wilde JJ, Eckersberg H, Suresh R, Tan X, Chen H, Farmer WT, Cha K, Le PU, Catoire H, Rochefort D, Kwan T, Yee BA, Dion P, Krishnaswamy A, Cloutier JF, Stifani S, Petrecca K, Yeo GW, Murai KK, Feng G, Rouleau GA, Ideker T, Sanjana NE, Zhou Y. Autism-associated CHD8 controls reactive gliosis and neuroinflammation via remodeling chromatin in astrocytes. Cell Rep. 2024 Aug 16;43(8):114637. doi: 10.1016/j.celrep.2024.114637.
Soubannier V, Chaineuau M, Deneault E, Gursu L, Lepine S, Kalaydjian D, Haghi G, Rouleau G, Durcan TM, Stifani S. Early nuclear phenotypes and reactive transformation in human iPSC-derived astrocytes from ALS patients with SOD1 mutations. Glia. 2024 Aug 2. doi: 10.1002/glia.24598.
Thiry, L., Sirois, J., Durcan, T.M. Stifani, S. Generation of human iPSC-derived phrenic-like motor neurons to model respiratory motor neuron degeneration in ALS. 2024. Commun Biol 7, 238. .
Tamaki Y, Ross J, Alipour P, Castonguay CE, Catoire H, Rochefort D, Urushitani M, Takahashi R, Sonnen J, Stifani S, Dion P, Rouleau G. Spinal cord extracts of amyotrophic lateral sclerosis spread TDP-43 pathology in cerebral organoids. 2023. PLoS Genet. Feb 6;(19):e1010606. doi: 10.1371/journal.pgen.1010606.
Tang Y, Pulimood N, Stifani S. Comparing the characteristics of microglia preparations generated using different human iPSC-based differentiation methods to model neurodegenerative diseases. 2022. ASN Neuro. 2022 Jan-Dec;14:17590914221145105. doi: 10.1177/17590914221145105.
